科学研究：

研究方向：

主要开展蔬菜作物，禾本科牧草及草坪草逆境生理和分子生物学研究。

承担的科研项目情况：

1)中国农大校聘特聘教授启动基金(2005-2008）主持。

2)国家留学回国基金(2006-2008）主持。

3)北京市农业局项目（2007-2009）主持。

4)教育部新世纪优秀人才支持计划,NCET-05-0133（2006-2008）主持。

5)“十一五”科技支撑计划，2007BAD57B04（2007-2010）技术骨干。

6)“十一五”科技支撑计划，2008BADB3B03（2009-2010）技术骨干。

7)国家重大基础研究973专项，2009CB119000（2009-2013）技术骨干。

8)国家自然基金，30871821（2009-2011）主持。

9)转基因生物新品种培育重大专项，2009ZX08005-024B（2009-10）课题主持。

10)高校博士点基金20100008110045（2011-2013）主持。

论文专著：

出版专著：

1. 植物细胞组织培养实验教程（主编）中国农业大学出版社ISBN 978-7-81117-879-1（2010），24万字。

2. 植物细胞组织培养 (参编) 中国农业大学出版社ISBN 978-7-81117-907-1（2010），52万字。

3. 滋补中药的生产与营销（副主编）中国农业出版社 ISBN 9787109-151338 （2011）40万字。

4. 黄瓜生产百问百答（主编）中国农业出版社 ISBN 978-7-109-12926-9（2008），25万字。

5. 新农村有机蔬菜生产实用手册（主编）人民出版社ISBN 978-7-01-008176-2（2009），31万字。

6. Pulli S. & Guo Y.D.* (2003) Rye microspore culture. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants. A manual. ISBN 1-4020-1544-5 Kluwer Academic Publisher, The Netherlands, p151-154.

7. Pulli S. & Guo Y.D.* (2003) Timothy androgenic culture. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants. A manual. ISBN 1-4020-1544-5 Kluwer Academic Publisher, The Netherlands, p173-178.

发表SCI论文：

1) Fu Q.S., Zhao B., Wang Y.J., Ren S., Guo Y.D. (2010) Stomatal development and associated photosynthetic performance of capsicum in response to differential light availabilities. Photosynthetica 48:189-198

2) Wang XW, Zhao B, Fu QS, Wang YJ, Ren S, Guo Y-D. The responses of morphological trait, leaf ultrastructure, photosynthetic and biochemical performance of tomato to differential light availabilities. Agricultural Sciences in China（Accepted）

3) Liu XW, Liu LY, Guo Y-D, Ren HZ (2011) Alleviating Effect of Exogenous Nitric Oxide in Cucumber Seedling against Chilling Stress. African Journal of Biotechnology (in press)

4) Niu JH, Jian H, Xu JM, Guo Y-D, Liu Q (2010) RNAi technology extends its reach: Engineering plant resistance against harmful eukaryotes. African Journal of Biotechnology. 9: 7573-7582

5) Ya Yu, Yong-Qin Zhao, Bing Zhao, Shuxin Ren, Yang-Dong Guo (2011) Influencing factors and structural characterization of hyperhydricity of in vitro regeneration in Brassica oleracea var. italica. Canadian Journal of Plant Science 91:1-7

6) Yu Y, Liu L-S, Zhao Y-Q, Yang P, Zhao B, Guo Y-D*（2010）A highly efficient in vitro plant regeneration and Agrobacterium-mediated transformation of Brassica oleracea var. botrytis. New Zealand Journal of Crop and Horticultural Science 38: (4) 1-11

7) Xing Y., Yu Y., Luo X., Zhang J.-N., Guo Y.-D. *（2010）High efficiency organogenesis and analysis of genetic stability of the regenerants in Solanum melongena. Biologia Plantarum 54 (2): 231-236

8) Guo YD, Hisano H, Shimamoto Y, Yamada T (2009) Transformation of androgenic-derived Festulolium plants (Lolium perenne L. x Festuca pratensis Huds.) by Agrobacterium tumefaciens. Plant Cell, Tissue and Organ Culture 96: 219-227

9) Yuan AP, Hou AB, Zhang FY & Guo YD* (2008) Inheritance and effects of the photoperiod sensitivity in foxtail millet (Setaria italica P. Beauv). Hereditas 145: 147-153

10) Qin Y., Li H.L., Guo Y.D.* (2007) High-frequency embryogenesis, regeneration of broccoli (Brassica oleracea var. italica) and analysis of genetic stability by RAPD. Scientia Horticulturae 111: 203-208

11) Qin Y., Gao L.H., Pulli S., Guo Y.D.* (2006) Shoot differentiation, regeneration of cauliflower and analysis of somaclonal variation by RAPD. Hereditas 143: 91-98

12) Guo Y.D., Mizukami Y. & Yamada T. (2005) Genetic characterization of androgenic progeny derived from Lolium perenne x Festuca pratensis cultivars. New Phytologist 166: 455-464

13) Ma R., Guo Y.D. & Pulli S (2004) A comparison of embryogenesis and regeneration between anther and microspore culture of rye (Secreal scale L.). Plant Cell, Tissue and Organ Culture 76:147-157

14) Guo Y.D. *., Yli-Mattila T. & Pulli S. (2003) Analysis of timothy (Phleum pratense L.) genetic variation with RAPD and UP-PCR. Hereditas 138: 101-113

15) Ma R, Guo Y.D. & Pulli S. (2003) Somatic embryogenesis and green plant regeneration from suspension cell-derived protoplasts of rye (Secale cereale L.). Plant Cell Reports 22:320-327

16) Guo Y.D. & Pulli S. (2001) Somatic embryogenesis and plant regeneration from suspension cultures of timothy (Phleum pratense L.). Acta Agriculture Scandinavica, Section B, Soil and Plant Science 51: 160-166

17) Guo Y.D.* & Pulli S. (2000) Isolated microspore culture and plant regeneration in rye (Secale cereale L.). Plant Cell Reports 19: 875-880

18) Guo Y.D., Niemelä T., Tulisalo U. & Pulli S. (2000) Maintenance of male sterile germplasm in Brassica rapa by in vitro propagation. Agricultural and Food Science in Finland 9: 231-238

19) Guo Y.D. & Pulli S. (2000) An efficient androgenic embryogenesis and plant regeneration through isolated microspore culture in timothy (Phleum pratense L.). Plant Cell Reports 19: 761-767

20) Guo Y.D. Sewón P. & Pulli S (1999) Improved embryogenesis from anther culture and plant regeneration in timothy. Plant Cell, Tissue and Organ Culture 57: 85-93

21) Guo Y.D. & Pulli S. (1996) High-frequency embryogenesis in Brassica campestris microspore culture. (1996) Plant Cell, Tissue and Organ Culture 46: 219-225

22) Guo Y.D. & Pulli S. (1995) In vitro pollen culture and the regeneration of Brassica campestris L. plants. Agricultural Science in Finland. 4: 513-518

其它论文：

1. 李蔚，刘莉莎，李仁，郭仰东（2010）十字花科蔬菜基因组含量的测定与分析。植物遗传资源学报 （已接收）

2. 王玉珏，付秋实，温常龙，程琳，赵冰，郭仰东（2010）干旱胁迫对黄瓜幼苗生长、光合生理特征及气孔特征的影响。中国农业大学学报 15(5) 12-18

3. Guo Y-D, Wang Y, Yang P, Liu L, Ren S (2010) RNAi-induced silencing of a cellulose synthase gene affects the leaf anatomy, cellulose content and drought tolerance in broccoli. 28th IHC, Lisbon, 20-28 Aug. 2010, Abstract Book p. 284

4. 程琳，温常龙，王玉珏，李仁，郭仰东，赵冰（2010）黑暗条件下不同贮藏方式和温度对辣椒穴盘苗质量的影响。北方园艺15: 156-159

5. 牛俊海; 卜祥霞; 薛慧; 赵海娟; 郭仰东; 简恒（2010）植物根结线虫基因组学研究进展。植物病理学报 40（3）225-234

6. 杨鹏，刘莉莎，温常龙，赵立群，赵冰，郭仰东（2010）青花菜ProDH基因的克隆及功能鉴定。基因组学与应用生物学29: 1-9

7. 温常龙，赵冰*，郭仰东*（2010）黑麦草与羊茅属间杂种研究进展。中国农业科学43 (7): 1346-1354 Wen CL, Zhao B, Yamada T, Guo Y-D (2010) Advances in festulolium intergeneric hybrid. Scientia Agricultura Sinica 43 (7): 1346-1354 (In Chinese with English abstract)

8. 王学文，付秋实，王玉珏，路河，郭仰东 (2010) 水分胁迫对番茄的生长、叶绿体超微结构及光合特性的影响. 中国农业大学学报15 (1)：7-13

9. 于 娅，刘莉莎，赵永钦，郭仰东* （2010）影响花椰菜农杆菌介导转化因素的研究。植物遗传资源学报 11: 320-325

10. 王颖，杨鹏，于娅，赵冰，郭仰东（2010）利用全测序基因组对被子植物纤维素合成酶基因的进化分析. 植物遗传资源学报 11（2）：179-185

11. 付秋实，李红岭，崔健，赵冰*，郭仰东* (2009) 水分胁迫对辣椒光合作用及相关生理特性的影响。中国农业科学 42（5）1859-1866 Fu QS, Li HL, Cui J, Zhao B, Guo Y-D (2009) Effects of water stress on photosynthetic characteristics of capsicum (Capsicum annuum L.). Scientia Agricultura Sinica 42（5）1859-1866 (In Chinese with English abstract)

12. 牛俊海，郭仰东，简恒（2009）RNA干扰在植物抗根结线虫病基因工程应用中的研究进展。基因组学与应用生物学28：167-173

13. 王学文，王玉珏，付秋实，赵冰*，郭仰东* （2009）弱光条件下番茄光合及生理特性与叶绿体超微结构的变化。华北农学报 24: (5) 144-149

14. 邢业，于娅，罗璇，安顺伟，郭仰东（2008）茄子下胚轴的再生研究及其遗传稳定性分析。华北农学报 23：17-21（增）

15. 罗璇，刘莉莎，于娅，邢业，赵冰，郭仰东（2008）山药类原球茎类似结构的发生及再生体系遗传稳定性分析。华北农学报23：27-30（增）

16. 付秋实，王学文，郑禾，郭仰东（2008）弱光对辣(甜)椒光合特性及光合启动时间的影响。华北农学报23：22-26（增）

17. 温贵聚; 赵泓; 郭仰东; 刘凡 （2008） 大白菜和黑芥种间杂种的获得及鉴定。华北农学报 23（4）105-109

18. 张继宁，袁文业，郭仰东（2007） 茄子主要农艺性状的相关与通径分析。中国农学通报 23（5）290-292

19. Yamada T, Guo YD, Mizukami Y, Tamura K, Tase K (2006) Introgression breeding program in Lolium/Festuca complex using androgenesis. Xu Zhihong et al (eds) Biotechnology and Sustainable Agricultire 2006 and Beyond. p447-450. Springer

20. Guo YD, Yamada T, Pulli S (2006) Improvement of forage grass breeding by androgenesis. 11th IAPTC&B Congress, Beijing, China, August 13-18. p189

21. Guo Y.D. & Yamada T. (2004) Androgenic embryogenesis and green plant regeneration in Festuca pratensis Huds. and Dactylis glomerata L. Breeding Research 6: (supplement) p259

22. Guo Y.D. & Yamada T. (2003) Androgenesis in forage grass breeding. Abstract book of the 2003 International Conference on Agricultural Science and Technology. Houston, Texas, USA. October 12-15, 2003. p17

23. Guo Y.D. & Yamada T. (2003) Androgenic embryogenesis and genome size variation in pollen-derived diploids and tetraploids in Festulolium. Breeding Research 5: (supplement) 225

24. Guo Y.D. & Yamada T. (2003) Preliminary report on androgenic embryogenesis and green plant regeneration of Lolium perenne L. x Festuca pratensis Huds. Grassland Science 49: 338-339

25. Ma R., Guo Y.D. & Pulli S. (2002) Development of doubled haploid lines in rye (Secale cereal L.) by anther culture. Current challenges in plant biology. Second SPPS Ph D student conference 2002, 8-11 August, Turku, Finland. p.29

26. Guo Y.D. & Pulli S. (2001) Somatic embryogenesis and plant regeneration from suspension culture of timothy. IX th International Turfgrass Research Conference. July 15-21, Toronto, Canada. Abstract Book. p.70.

27. Guo Y.D., Yli-Mattila T. & Pulli S. (2001) Assessment of genetic diversity in Phleum pratense L. using RAPD marker. The Phytochemical Society of Europe, Plant Biotechnology Workshop 10-13 June, 2001 Helsinki, Finland. Abstract Book p.38

28. Pulli S. & Guo Y.D. (2001) Biotechnological improvement in timothy breeding. Proceedings of the XIX international grassland congress. 11-21 February, 2001, São Paolo, Brazil. pp 494-495

29. Pulli S. & Guo Y.D. (1999) Haploid production in turnip, timothy and rye. XVI International Botanical Congess 1-7 August 1999 Saint Louis, Missouri, U.S.A. Abstract Book p.125

30. Pulli S. & Guo Y.D. (1998) Androgenic embryogenesis from microspore culture in rye (Secale cereale L.). 5th Plant Embryogenesis Workshop and 2nd European Plant Embryogenesis Network Meeting. Barcelona, 19-21, Nov. 1998. Abstract Book. p. 78

31. Pulli S., Guo Y.D. & Sewon P. (1998) Induction of haploids from anther and microspore culture of timothy (phleum pratense L.) 20th Bioscience Days (6th Meeting of Finnish Plant Scientists) 17-19 September, 1998. Helsinki. p. 46

32. Pulli S., Guo Y.D. & Sewon P. (1998) Anther culture and plant regeneration of timothy (phleum pratense L.). 17th General Meeting of the European Grassland Federation, 18-21, May 1998. Debrecen, Hungary. Abstract Book. p. 8-9

33. Guo Y.D. & Pulli S. (1997) Induction of haploid plants from isolated pollen of Brassica campestris. 10th Crucifer Genetics Workshop, ISHS Symposium on Brassicas. 23-27, September 1998. Rennes, France. Abstract Book. p.163

34. Guo Y.D. & Pulli S. (1995) Preliminary report on embryogenesis of Brassica rapa L. ssp oleifera via in vitro isolated microspore culture. 14th Eucarpia Congress. 31, July-4, August, 1995. Jyväskylä, Finland. Abstract Book. p.97

35. 郭仰东 (1994) 冷处理对提高黄瓜抗寒性的研究。 园艺学报 21: 101-102

36. Li XO, Sun RF, Wu FY, Si JG, Nie XK, Li CL, Jiang ZR, Guo YD, Ye BJ (1995) In vitro propagation of Chinese cabbage for seed production. Acta Horticulturae. 402: 306-312

37. 郭仰东 (1993) 韭菜雄性不育株的发现及利用。 蔬菜 66: 18

38. 郭仰东,李春玲,蒋钟仁(1992)用组培方法繁殖韭菜雄性不育无性系的研究简报。 北京农学院学报 7: 85-87

荣誉奖励：

1.2002年获日本学术振兴会(JSPS)研究计划。

2.2005年获教育部新世纪优秀人才支持计划。

3.2006年获中国农业大学优秀班主任。

4.2007年获中国农业大学优秀教师。